Composite
Part:BBa_K2558205:Design
Designed by: Tsinghua 2018 Group: iGEM18_Tsinghua (2018-10-05)
Ptac test device with Anderson weak-expressed lacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1073
Illegal NheI site found at 1096 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2277
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 176
Design Note
In order to investigate how lacI dosage affects IPTG induction, we used Anderson promotor J23100, J23110 and J23114 to design three constitutive lacI generators of different intensities. The three lacI generators were then ligated with Ptac driven reporter sfGFP to make three IPTG induction devices (BBa_K2558203, BBa_K2558204, BBa_K2558205). By measuring sfGFP fluorescence we tested how these devices react to IPTG.