Part:BBa_K1291571
HORSERADISH PEROXIDASE TYPE C
The enzyme horseradish peroxidase (HRP), found in the roots of plant horseradish, is used extensively in biochemistry applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. It is a metalloenzyme with many isoforms and the most studied type is C. This enzyme is primerly responsible for the degradation of industrial dyes such as Methylene Blue.
HRP Background, Usage and Biology
Horseradish peroxidase (HRP) is an enzyme used in molecular biology. It can be used in various biological methods, such as ELISA, Western Blotting, Southern Blotting, and EMSA [1]. HRP’s colorimetric and chemiluminescent properties make it a useful agent in many tests. HRP acts as a catalyst in redox reactions [3]. A luminol and H2O2 solution can release light for a very long time, but when HRP is added, it usually reaches it maximum light emission between 1 and 5 minutes [4]. This makes it a useful detection method. HRP can be used in more qualitative methods as well, since it induces changes in color when used with chromogenic assays. Commonly used substrates for this type of detection are 3,3',5,5'-tetramethylbenzidine (TMB), 2,2' -azino-di-[3-ethylbenzthiazoline-6-sulfonic acid] (ABTS), and diaminobenzidine (DAB) [3,4]. HRP when added to TMB, for example, yields a 2-electron oxidation product which yields a blue color. HRP is also up and coming in the medical field. When combined with other agents, it is heavily reactive toward tumor cells in humans [2]. It also serves as a functional tracer. HRP can be injected into the bloodstream and then identified at different places in the pathway using the DAB/H2O2 reaction [4]. This makes HRP very useful for immunocytochemistry and electron microscopy [4].
References
[1] https://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=15552120 [2]https://chem.libretexts.org/Textbook_Maps/Biological_Chemistry/Catalysts/Case_Studies/Horseradish_Peroxidase [3] http://www.bio-rad.com/featured/en/hrp-substrate.html [4] https://www.sciencedirect.com/topics/neuroscience/horseradish-peroxidase [5] http://www.jbc.org/content/257/7/3669.full.pdf
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 384
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 504
Illegal AgeI site found at 658
Illegal AgeI site found at 961 - 1000COMPATIBLE WITH RFC[1000]
| None |