Composite

Part:BBa_K2533053:Design

Designed by: kangyuan yu   Group: iGEM18_HUST-China   (2018-09-21)
Revision as of 15:31, 17 October 2018 by MrK (Talk | contribs) (→‎Source)


RBS-mleS-RBS-lldP-RBS-ldhA-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 212
    Illegal BglII site found at 1942
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 529
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 904
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 1698
    Illegal NgoMIV site found at 2064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 936
    Illegal BsaI site found at 2435
    Illegal BsaI.rc site found at 388
    Illegal BsaI.rc site found at 676
    Illegal BsaI.rc site found at 1243
    Illegal BsaI.rc site found at 1588
    Illegal BsaI.rc site found at 2331
    Illegal BsaI.rc site found at 4313


Design Notes

Compared with cyanobacteria, we find Rhodopseudomonas palustris is more suitable than cyanobacteria to provide Lactate to Shewenella due to its charactestics of Anaerobic respiration.Therefore, we decided to modify Rhodopseudomonas palustris so that it could produce lactate under the anaerobic condition and transport lactate to the extracellular. And we have found that if we want to enhance the production of lactate from Rhodopseudomonas palustris, we can promote the convertion efficiency of pyruvate to D-lactate and malate to L-lactate. Therefore, we decided to use this two genes, mleS and ldhA to help Rhodopseudomonas palustris to produce lactate. Also, Considering the necessity of helping Rhodopseudomonas palustris to transport lactate out of the membrane, we need to add a lldP gene. TT is a common terminator.

Source

Lactococcus lactis subsp. lactis Ⅱ1403 & Escherichia coli str. K-12 substr. MG1655 & Escherichia coli str. K-12 substr. MG1655

References