Composite

Part:BBa_K2622033

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)
Revision as of 15:28, 17 October 2018 by GAMBIT (Talk | contribs)

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His-igA

His-IgA has a 6x His-tag fused to translocator domain's (BBa_K2622003) N-terminus, so that it could be displayed and detected in the outer surface of the membrane. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has incompatible XbaI, EcoRI and PstI sites that must be avoided when cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 198
    Illegal XbaI site found at 39
    Illegal PstI site found at 181
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 198
    Illegal NheI site found at 108
    Illegal PstI site found at 181
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 198
    Illegal BglII site found at 177
    Illegal BamHI site found at 164
    Illegal XhoI site found at 173
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 198
    Illegal XbaI site found at 39
    Illegal PstI site found at 181
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 198
    Illegal XbaI site found at 39
    Illegal PstI site found at 181
    Illegal NgoMIV site found at 1041
    Illegal AgeI site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


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