Composite

Part:BBa_K2533055:Design

Designed by: Ziyang Xiao   Group: iGEM18_HUST-China   (2018-09-28)
Revision as of 15:23, 17 October 2018 by MrK (Talk | contribs) (→‎Source)


RBS-ldhA-RBS-lldP-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 1065
    Illegal NgoMIV site found at 1431
    Illegal NgoMIV site found at 1503
    Illegal NgoMIV site found at 1572
    Illegal NgoMIV site found at 1706
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1802
    Illegal BsaI.rc site found at 982
    Illegal BsaI.rc site found at 1698


Design Notes

Compared with cyanobacteria, we find Rhodopseudomonas palustris is more suitable than cyanobacteria to provide Lactate to Shewenella due to its charactestics of Anaerobic respiration.Therefore, we decided to modify Rhodopseudomonas palustris so that it could produce lactate under the anaerobic condition and transport lactate to the extracellular. And we have found that if we want to enhance the production of lactate from Rhodopseudomonas palustris, we can promote the convertion efficiency of pyruvate to D-lactate and malate to L-lactate. Therefore, we decided to use this two genes, mleS and ldhA to help Rhodopseudomonas palustris to produce lactate. Also, Considering the necessity of helping Rhodopseudomonas palustris to transport lactate out of the membrane, we need to add a lldP gene. In this part, we switch the composition of lldp and ldha to detect the production of Lactate. TT is a common terminator.

Source

Escherichia coli str. K-12 substr. MG1655 & Escherichia coli str. K-12 substr. MG1655

References