Composite

Part:BBa_K2533047:Design

Designed by: kangyuan yu   Group: iGEM18_HUST-China   (2018-09-15)
Revision as of 14:45, 17 October 2018 by MrK (Talk | contribs) (→‎Design Notes)


RBS-ldhA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
    Illegal NgoMIV site found at 457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 982


Design Notes

Compared with cyanobacteria, we find Rhodopseudomonas palustris is more suitable than cyanobacteria to provide Lactate to Shewenella due to its charactestics of Anaerobic respiration.Therefore, we decided to modify Rhodopseudomonas palustris so that it could produce lactate under the anaerobic condition and transport lactate to the extracellular. And we have found that if we want to enhance the production of lactate from Rhodopseudomonas palustris, we can promote the convertion efficiency of pyruvate to D-lactate and malate to L-lactate. Therefore, we decided to use this two genes, mleS and ldhA to help Rhodopseudomonas palustris to produce lactate.

Source

Escherichia coli str. K-12 substr. MG1655

References