Part:BBa_K2595007
RprA
RprA is a small regulatory RNA that is required for wild-type production of RpoS (RNA polymerase, sigma S) (Majdalani et al., 2001). Binding of RprA to Hfq is required for stability and activity in vivo; it binds upstream of the base pairing region within rpoS mRNA (Soper et al., 2010).
RprA is also involved in negative regulation of biofilm formation (by repressing expression of CsgD). It does so through direct binding of RprA to the 5'-untranslated and translational initiation regions of csgD mRNA, inhibiting translation and promoting csgD mRNA degradation (Mika et al., 2012).
Usage and Biology
This synthetic version of RprA follow the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RprA can regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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