Coding

Part:BBa_K2789026

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)
Revision as of 14:21, 17 October 2018 by Talent b (Talk | contribs)


Lectin-FimH

Similar to K2789024, this part is also display the lectin to the surface of the bacteria. Lectin is a protein that originally from Green algae. It has positive charge thus can bind with EPS(exopolysubstance),which is secreted by algae. In our project, we wanted our bacteria to connect with the algae to strengthen the symbiotic biofilm. Thus we wanted to make it expressed on the surface of bacteria. Fusing lectin with FimH is another way to display it on bacteria surface. FimH is a part of pili，some teams had identified that make fused protein with this protein can make the target protein expressed on the surface.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 442
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 442
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 442
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 442
    Illegal AgeI site found at 106
    Illegal AgeI site found at 244
    Illegal AgeI site found at 345
    Illegal AgeI site found at 600
    Illegal AgeI site found at 742
    Illegal AgeI site found at 1045
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None