Coding

Part:BBa_K2665016

Designed by: You Tong   Group: iGEM18_Kyoto   (2018-10-08)
Revision as of 14:04, 17 October 2018 by Sima914 (Talk | contribs)


TDH3-AtHKT1-6xHis-CYC

HKT family proteins are high K+ affinity transporters, and AtHKT1 is expressed in xylem of Arabidopsis thaliana. While HKT1 from wheat transport K+, AtHKT1 does not transport K+. Otherwise, AtHKT1 is better in Na+ transport. AtHKT1 inhibits the growth of S. cerevisiae in certain salt concentration.So, it probably transport Na+ into yeast cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2238
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1376

Reference

[4] A. Y. Ryss, O. A. Kulinich, and J. R. Sutherland, “Pine wilt disease: a short review of worldwide research,” For. Stud. China, vol. 13, no. 2, pp. 132–138, Jun. 2011.
[5] Y. Mamiya, “History of Pine Wilt Disease in Japan 1,” J. Nematol., vol. 20, no. 2, pp. 219–226, 1988.


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