Part:BBa_K2595005
RyhB
RyhB is a 90 nucleotides-long, non-coding RNA with AU-rich regions. RyhB RNA down-regulates the quantity of iron-using proteins when there is limitation on iron hence regulating a set of iron-storage. The structure of the RyhB RNA is critical for its function and regulation. In E.Coli, the AU-rich region of RyhB binds to Hfq, the RNA chaperone, to target mRNA sodB and result in changes in mRNA transcription, translation or stability. RyhB binding to the Hfq protein is essential in increasing its interaction with its target messages.
Usage and Biology
This synthetic version of RyhB follows the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RyhB can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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