Coding

Part:BBa_K2684005

Designed by: Yihao Zhang   Group: iGEM18_SHSBNU_China   (2018-10-08)
Revision as of 13:34, 17 October 2018 by JimLi2002 (Talk | contribs)


SpyCatcher-CotA

SpyCatcher fused with CotA by 2xGGGGS linker

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 841


We added the SpyCatcher sequence after cotA gene so that we can produce laccase protein with spycatcher (BBa_K2684005).

[edit]
Categories
Parameters
None