Part:BBa_K2595009

SgrS

SgrS (wt) is a small regulatory sRNA that controls the expression of the PtsG gene in Escherichia coli K-12. The PtsG gene codes for the major glucose transporter, which is necessary for the transport of glucose into the cytoplasm. SgrS action is dependent of Hfq, a chaperone that interacts with the sRNA at its hairpin loop and poly U tail. This stabilizes the interaction of the 3’ end of SgrS with the Shine Dalgarmo sequence of ptsG mRNA by partial complementary base-pairing (Bobrovskyy and Vanderpool, 2014). Upon successful formation of the sRNA-mRNA duplex complex, the formation of the ribosomal machinery is inhibited, therefore blocking translation.

Usage and Biology

This synthetic version of SgrS follow the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, SgrS can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]