Device

Part:BBa_K2719009:Design

Designed by: Andrea Pamela Jimenez Tapia & Rodrigo Valencia Ocampo   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 13:03, 17 October 2018 by AKARAM (Talk | contribs) (→‎Design Notes)


Leptin Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed by a T7-lac promoter (BBa_R0184), a RBS binding site, a leptin coding region and a double terminator. Also, it was optimized for being cloned in pSB1C3 and transformed in E. coli BL21 (DE3). This construct allows for an IPTG controlled expression of leptin.

Source

Synthetic part.

References