Coding

Part:BBa_K2622006

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)
Revision as of 12:44, 17 October 2018 by GAMBIT (Talk | contribs)


Mistic (Mstx)

Mistic (Mstx) is an unique hydrophilic protein from Bacillus subtilis that works as a fusion tag and helps to integrate other proteins into lipid membranes more easily. When fused to the N-terminus of integral membrane proteins, MstX enables the cargo proteins to fold into their native conformations in the membrane, thus yielding a high-level expression. Therefore, this biobrick has RFC12 prefix and suffix that allows to fuse proteins to the N or C-terminus in a stop codon free way.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None