Coding

Part:BBa_K2622004

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)
Revision as of 11:18, 17 October 2018 by GAMBIT (Talk | contribs)


scFv_antiVGY

Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.

This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41


[edit]
Categories
//collections/immune_regulation/antibodies
Parameters
None