Coding

Part:BBa_K2719002

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 10:53, 17 October 2018 by JRUEDA (Talk | contribs)


GST+Tenascin Domain V

This part is the fusion between both proteins and that helps us to simplify tenascin purification. GST has tenascin fused on the N-terminal. The binding was formed by a polyproline linker that gives tenascin three dimensional structure and stability. For more information see <a href="https://parts.igem.org/Part:BBa_K2719001">BBa_K2719001</a> and <a href="https://parts.igem.org/Part:BBa_K2719003">BBa_K2719003</a>.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 346


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Parameters
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