Composite

Part:BBa_K2569031:Design

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-10-04)
Revision as of 10:52, 17 October 2018 by DDY (Talk | contribs) (→‎Design Notes)

PdnaA -NicA2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nicotine oxidase NicA2 behind our strongest constitutive promoter leading to enhance nicotine degradationin Pseudomonadaceae compared to original nicA2 gene.

Source

References