Regulatory

Part:BBa_K2569023:Design

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-09-29)
Revision as of 10:15, 17 October 2018 by DDY (Talk | contribs)

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Pfat, constitutive promoter in Pseudomonas. fluorescence pf-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter Pfat is a strong constitutive promoter that can be used in regulating the gene expression in Pseudomonadaceae.

Source

Its sequence is taken from the upsteam of the gene fat acid by artificially sythesis.

References