Regulatory

Part:BBa_K2569021:Design

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-09-29)
Revision as of 10:10, 17 October 2018 by DDY (Talk | contribs)

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Pfme, constitutive promoter in Pseudomonas. fluorescence pf-5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 63
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 63
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 63
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 63
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter Pfme is a strong constitutive promoter that can be used in regulating the gene expression in Pseudomonadaceae.

Source

Its sequence is taken from the upsteam of the gene fme by artificially sythesis.

References