Coding

Part:BBa_K2596005:Design

Designed by: Matthew Mullin   Group: iGEM18_Stony_Brook   (2018-10-10)
Revision as of 08:52, 17 October 2018 by Kledalla (Talk | contribs) (→‎Source)


cscB (sucrose permease) from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized cscB for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through a silent point mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon.

Source

E. coli

References

Qiao C, Duan Y, Zhang M, et al. Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942. Appl Environ Microbiol. 2018;84(2): e02023-17. doi: 10.1128/AEM.02023-17.

McEwen JT, Machado IMP, Connor MR, et al. Engineering Synechococcus elongatus PCC 7942 for Continuous Growth under Diurnal Conditions. Appl Environ Microbiol. 2013;79(5): 1668-1675. doi: 10.1128/AEM.03326-12.