Coding

Part:BBa_K2596005

Designed by: Matthew Mullin   Group: iGEM18_Stony_Brook   (2018-10-10)
Revision as of 08:52, 17 October 2018 by Kledalla (Talk | contribs)


cscB (sucrose permease) from E. coli

This is a sucrose symporter protein from E. coli.

Usage and Biology

CscB is a cell membrane protein that plays an integral role in transporting sucrose across the membrane as it is a sucrose-proton symporter. It is compatible in many strains of Synechococcus elongatus, including 2973, 6803 and 7942. S. elongatus is unique in that its cell membrane proton gradient is generated by maintaining a surplus of protons inside the cell. Sucrose can then be exported from the cell using the proton gradient.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


Sucrose Assays

For experimental methods and results on characterization, look at the page for composite part BBa_K2596015.

[edit]
Categories
//cds/membrane/pump
//chassis/prokaryote/cyanobacterium
Parameters
biologySynechococcus elongatus PCC 7942
device_typeCoding sequence