Regulatory

Part:BBa_K2596003:Design

Designed by: Priya Aggarwal, Lin Yu, Natalie Lo   Group: iGEM18_Stony_Brook   (2018-10-09)
Revision as of 08:45, 17 October 2018 by Kledalla (Talk | contribs) (→‎References)


PpsbA2 from Synechococcus elongatus PCC 7942


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Included light binding regions


Source

Synechococcus elongatus PCC 7942

References

Agrawal GK, Kato H, Asayama M, et al. An AU-bx motif upstream of the SD sequence of light-dependent psbA transcripts confers mRNA instability in darkness in cyanobacteria. Nucleic Acids Research. 2001;29(9): 1835-1843. doi: 10.1093/nar/29.9.1835.

Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol. 2000;305:527-42. PubMed 10812624

Englund E, Liang F, Lindberg P. Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Scientific Reports. 2016;6(36640): 1-12. doi: 10.1038/srep36640.

Koksharova, OA, Klint J, Rasmussen U. The first protein map of Synechococcus sp. Strain PCC 7942. Microbiology. 2006;75: 664-672. doi: 10.1134/S0026261706060087.

Kulkarni RD, Golden SS. Adaptation to High Light Intensity in Synechococcus sp. Strain PCC 7942: Regulation of Three psbA Genes and Two Forms of the D1 Protein. J. Bacteriol. 1994;176(4): 959-965. doi: 10.1128/jb.176.4.959-965.1994.