Composite

Part:BBa_K2611010

Designed by: Qinling Qiu   Group: iGEM18_SCU-China   (2018-10-17)
Revision as of 08:03, 17 October 2018 by Qinling (Talk | contribs)


sgRNA(spacer J23101-GFP)-spacer J23101-GFP

We added a spacer sequence and a NGG site closely before the promoter (<a href="https://parts.igem.org/Part:BBa_J23101">'J23101'</a>). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.We have submitted the sgRNA part (BBa_K2611000) and spacer-GFP part(BBa_K2611001). In this composite part, we linked them together.We observed positive colonies transformed with sgRNA(spacer J23101-GFP)-spacer J23101-GFP(part BBa_K2611010) under a stereo fluorescence microscope.Green fluorescence was observed.(Fig.1)

Long description.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 226
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 924


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Categories
Parameters
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