Coding

Part:BBa_K2710002

Designed by: Brian Ee   Group: iGEM18_UNSW_Australia   (2018-10-05)
Revision as of 07:53, 17 October 2018 by Emilywatson (Talk | contribs)


His-Alpha Prefoldin with SpyCatcher

6xHis Alpha Prefoldin with Spy-Catcher


Usage and Biology

Alpha prefoldin (aPFD) (15.7 kDa) and beta prefoldin (bPFD) (13.8 kDa) derived from the thermophilic arcahea Methanobacterium thermoautotrophicum oligomerise to form a heterohexameric structures (87 kDa), consisting of two alpha subunits and four beta subunits1. The prefoldin hexamer is assembled through interactions between beta hairpins in each subunit. These beta hairpins form two 8 stranded up and down beta barrels. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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