Coding

Part:BBa_K2710002

Designed by: Brian Ee   Group: iGEM18_UNSW_Australia   (2018-10-05)
Revision as of 07:40, 17 October 2018 by Emilywatson (Talk | contribs)


His-Alpha Prefoldin with SpyCatcher

6xHis Alpha Prefoldin with Spy-Catcher


Usage and Biology

Alpha prefoldin (aPFD) (15.7 kDa) and beta prefoldin (bPFD) (13.8 kDa) derived from Methanobacterium thermoautotrophicum oligomerise to form heterohexameric structures (87 kDa), consisting of 2 alpha subunits and 4 beta subunits2. The prefoldin hexamer is assembled through interactions between beta hairpins in each subunit. These beta hairpins form two 8 stranded up and down beta barrels. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None