Regulatory

Part:BBa_K2876000

Designed by: Isaac Justice   Group: iGEM18_Stanford   (2018-06-22)
Revision as of 06:48, 17 October 2018 by Ijustice (Talk | contribs)


placOL2-62 E. coli promoter

This is an E. coli lacZ promoter with a modified upstream lambda-cl bonding site used for transcription in a E. coli two hybrid system. The sequence for this promoter was donated by Ann Hochschild

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None