Reporter

Part:BBa_K2611001

Designed by: Changhe Li   Group: iGEM18_SCU-China   (2018-10-17)
Revision as of 06:26, 17 October 2018 by White (Talk | contribs)


spacer J23101-GFP 

   We added a spacer sequence and NGG site closely before the promoter (BBa_B0034). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.

To be used in pSB1C3. 

   We selected part J23101-GFP (BBa_J364000) as a reporter to test the function of the modified promoter and measure the repression level. The modified promoter does not influence the function of GFP gene as the GFP fluorescence intensity of the modified part is even stronger than the original one (Figure 3).


SCU China-2018 spacer J23101 GFP.png

SCU China-2018 spacer J23101 GFP(2).png

SCU China-2018 spacer J23101 GFP(3).png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 30
    Illegal NheI site found at 53
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 728


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Parameters
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