Coding

Part:BBa_K2862014

Designed by: Thomas Caganek   Group: iGEM18_Imperial_College   (2018-10-09)
Revision as of 06:20, 17 October 2018 by JoshLawrence96 (Talk | contribs)


SoxR from E. coli (new)

SoxR from E.coli (new)

SoxR is a redox-sensitive transcription factor providing transcriptional activation or repression downstream of the pSoxS promoter when oxidised, either directly by redox-cycling drugs or by oxidative stress. It is therefore inducible by various redox-cycling drugs, toxins, antibiotics, heavy metals, hydrogen peroxide and nitric oxide: providing various applications in the development of environmental and therapeutic devices. By coupling oxidation of redox-cycling species to an electrode, the 2018 Imperial College London iGEM team (PixCell) used SoxR to build electrogenetic devices where electrical inputs modulated gene expression.

This SoxR CDS was taken from X, and is codon optimised for E. coli. It forms part of the PixCell library of electrogenetic and redox-sensing parts.

This part has been designed to be fully modular, being compatible for BioBrick, BASIC and Golden Gate assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal BsaI.rc site found at 532


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Categories
Parameters
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