Part:BBa_K2753012:Design

pSB1C3-pRPL18P promoter-CrISY-tTDH1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 701
  Illegal BglII site found at 728
  Illegal XhoI site found at 1884
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

None.

Source

We ordered the part from a synthesis company, GenScript.

References

[1]: Sherden, N. H., Lichman, B., Caputi, L., Zhao, D., Kamileen, M. O., Buell, C. R., & Connor, S. E. O. (2018). Identification of iridoid synthases from Nepeta species: Iridoid cyclization does not determine nepetalactone stereochemistry, 145, 48–56. https://doi.org/10.1016/j.phytochem.2017.10.004
[2]: Billingsley, J. M., Denicola, A. B., Barber, J. S., & Tang, M. (2017). Engineering the biocatalytic selectivity of iridoid production in Saccharomyces cerevisiae. Metabolic Engineering, 44(August), 117–125. https://doi.org/10.1016/j.ymben.2017.09.006
[3]: Campbell, A., Bauchart, P., Gold, N. D., Zhu, Y., De Luca, V., & Martin, V. J. J. (2016). Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids. ACS Synthetic Biology, 5(5), 405–414. https://doi.org/10.1021/acssynbio.5b00289
[4]: Lee, M. E., Deloache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly-characterized Yeast Toolkit for Modular , Multi-part Assembly. https://doi.org/10.1021/sb500366v