Composite

Part:BBa_K2753012

Designed by: WEI KUANGYI   Group: iGEM18_GreatBay_China   (2018-08-29)
Revision as of 05:39, 17 October 2018 by CharlesW (Talk | contribs)


pSB1C3-pRPL18P promoter-CrISY-tTDH1

Catharanthus roseus ridoid synthase (ISY) codon-optimised for use in S. cerevisiae catalysing the reductive cyclisation to produce nepetalactol. Expression of this circuit is regulated by a yeast constitutive promoter pRPL18B and terminator tTDH1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
    Illegal BglII site found at 728
    Illegal XhoI site found at 1884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None