Translational_Unit

Part:BBa_K2705005

Designed by: Danqing Tong   Group: iGEM18_NKU_CHINA   (2018-09-30)
Revision as of 05:19, 17 October 2018 by Tong Danqing (Talk | contribs)


PgltAB-GFP

This part includes the promoter gltA and gltB and GFP, the expression of GFP is repressed by high level cellular glutamate because gltA and gltB is upregulated by GltC, which is repressed by cellular glutamate level. A restriction enzyme KpnI cutting site is between gltA and gltB and GFP. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
    Illegal XhoI site found at 572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None