Composite

Part:BBa_K2683030

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)
Revision as of 04:00, 17 October 2018 by Kristiturton (Talk | contribs)

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Peptidyl Prolyl Isomerase (PPiase) in pSB1C3

Peptidyl-prolyl isomerase (PPiase) is a protein that aids the isomerization of prolines in order to catalyze the addition proline amino acids onto the peptide chains from the aminoacyl-tRNA it is attached to [1]. This part has been codon optimized for use in E.coli , Contains a N-terminal his tag, and has a T7 promoter, medium strength RBS, and double terminator. This part was originally designed by the 2017 LEthbridge iGEm team (http://2017.igem.org/Team:Lethbridge).

This part was improved by placing it into the pSB1C3 plasmid.

T--Lethbridge--PPiaseOE.PNG

Figure 1- An overexpression gel of PPiase protein run on a 12% SDS-PAGE. Samples were taken at point of induction and then in 1 hour increments thereafter. The induced proteins is expected to be seen at about 22kDa range

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None