Part:BBa_K575026

LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0030) + LasR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and an RFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 58
  Illegal NheI site found at 907
  Illegal NheI site found at 930
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1286
  Illegal AgeI site found at 741
  Illegal AgeI site found at 853
  Illegal AgeI site found at 1483
  
• 1000
  COMPATIBLE WITH RFC[1000]

[http://2018.igem.org/Team:RMHS_Maryland Team RMHS_Maryland 2018] contributed to the characterization of this part by demonstrating that the part produces RFP expression when induced by AI-1.
(--ishayardi 20:24, 16 October 2018 (UTC))