Other

Part:BBa_J72004:Design

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)
Revision as of 05:32, 16 July 2008 by JCAnderson (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

{phi80-att} phage integration site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 411
    Illegal SpeI site found at 164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1183F/ca1183R on pG80 CRIM plasmid   (BglII/XhoI)
Sub into pBca9145-Bca1144#5                (BglII/XhoI)
Product is pBca9145-Bca1183
----
ca1183F	BBb biobricking of phi80 att
ctctgAGATCTcatgggcccgtgcgaatcag
ca1183R	BBb biobricking of phi80 att
gcaaaCTCGAGttaGGATCCgctagctgataacgggaaag

References