Coding

Part:BBa_K2812006:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brls   Group: iGEM18_TU-Eindhoven   (2018-10-03)
Revision as of 00:55, 17 October 2018 by GuidoOerlemans (Talk | contribs) (→‎Design Notes)


Coding sequence for Pyocin S5 with HlyA and His6-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal BglII site found at 425
    Illegal BglII site found at 2110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1200


Design Notes

Original DNA sequence based on Pseudomonas aeruginosa PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985). This DNA sequence is codon optimized for E. coli.

Source

Genomic sequence from Pseudomonas aeruginosa. DNA construct produced by IDT.

References

Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.