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Part:BBa_J72003:Design

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)
Revision as of 05:26, 16 July 2008 by JCAnderson (Talk | contribs) (→‎Source)

{R6K} conditional origin of replication in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1182F/ca1182R on pG80 CRIM plasmid (BglII/XhoI) Sub into pBca9145-Bca1144#5 (BglII/XhoI,L) Product is pBca9145-Bca1182

ca1182F BBb Biobricking of R6K ctctgAGATCTaGCAGTTCAACCTGTTGATAG ca1182R BBb biobricking of R6K gcaaaCTCGAGttaGGATCCgtgattcgcacgggcccatg

References