Coding

Part:BBa_K2683006

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)
Revision as of 00:30, 17 October 2018 by Kristiturton (Talk | contribs)


FitD Codon optimized for E.coli

FitD is a protein that is used as molluscicidal toxin. FitD is originally derived from Psuedomonas protegens . This protein has been recognized as a treatment for zebra and quaga mussel populations which are a invasive species in North America[1]. This sequence has been codon optimized for expression in E.coli .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2529
    Illegal BglII site found at 2721
    Illegal BamHI site found at 2175
    Illegal BamHI site found at 2277
    Illegal BamHI site found at 2808
    Illegal BamHI site found at 4290
    Illegal BamHI site found at 6462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 435
    Illegal NgoMIV site found at 1968
    Illegal NgoMIV site found at 2694
    Illegal NgoMIV site found at 2874
    Illegal NgoMIV site found at 2979
    Illegal NgoMIV site found at 3027
    Illegal NgoMIV site found at 4638
    Illegal AgeI site found at 3714
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None