Regulatory

Part:BBa_K108014:Experience

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)
Revision as of 23:10, 16 October 2018 by Owen-lo 923 (Talk | contribs) (→‎User Reviews)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K108014

User Reviews

UNIQ3f68d6e8f49c91ea-partinfo-00000000-QINU UNIQ3f68d6e8f49c91ea-partinfo-00000001-QINU

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Edinburgh OG 2018

We access the online NCBI data (NCBI: AM260479.1) and use reference 1 as guideline and found out the current available the functional promoter is being 134bp shorter. Therefore we have used the new phaR and promoter sequence as our new template. Due to the excessive GC in the sequence, we have it codon optimised to E.coli and generated the part through the IDT company service.

Reference 1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.

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