Coding

Part:BBa_K2765021:Design

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-09-15)
Revision as of 22:55, 16 October 2018 by Llj (Talk | contribs)

To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by gal1 promoter, and thus the target gene is induced by galactose and repressed by glucose.

T--BIT-China--PartRegulator_yno1_Fig4_Size.png

Fig.4 The size of yno1 (1713bp)


T--BIT-China--PartRegulator_yno1_Fig5_Plasmid.png

Fig.5 plasmid of yno1 expression


T--BIT-China--PartRegulator_yno1_Fig6_ColonyPCR.png

Fig.6 result of Colony PCR

1~7: negative results

8: positive result (yno1 1713bp)