Composite

Part:BBa_K2637052:Design

Designed by: Yiran Liu   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 21:53, 16 October 2018 by Old-child (Talk | contribs) (→‎Design Notes)


mCherry with TEF1 Promoter and TEF1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 266


Design Notes

We added the constitutive TEF1 promoter and TEF1 terminator to the Part:BBa_E2060 to make the cassette which can express mCherry continously.

Source

The gene mCherry derives from Part:BBa_E2060. The PGK1 promoter and PGK1 terminator are from saccharomyces cerevisiae.


References