Part:BBa_K209023

Aar1 C-D part, FKBP

dimerizes with FRB when rapamycin is present

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

2018 Peking Team improvement

Team Peking 2018 improved this part.

Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601008

Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601025 https://parts.igem.org/Part:BBa_K2601026 https://parts.igem.org/Part:BBa_K2601027

Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601011

Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.

https://parts.igem.org/Part:BBa_K2601037 https://parts.igem.org/Part:BBa_K2601038