Composite

Part:BBa_K2637049:Design

Designed by: Jiangnan Xie   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 21:00, 16 October 2018 by Old-child (Talk | contribs) (→‎Design Notes)

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EYFP cassette with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1717


Design Notes

We added the constitutive TDH3 promoter and ADH1 terminator to the Part:BBa_E2030 to make the cassette which can express EYFP continously.

Source

The gene EYFP derives from Part:BBa_E2030. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.


References