Composite

Part:BBa_K2637048:Design

Designed by: Jiangnan Xie   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 20:33, 16 October 2018 by Old-child (Talk | contribs)


Fluc with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1119
    Illegal NgoMIV site found at 2463
    Illegal NgoMIV site found at 2484
    Illegal AgeI site found at 2187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2369


Design Notes

We connected Fluc with constitutive TDH3 promoter and ADH1 terminator to make the cassette which can express Fluc continuously.


Source

111

References