Composite

Part:BBa_K2739009:Design

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-09)
Revision as of 20:21, 16 October 2018 by Owen-lo 923 (Talk | contribs)


Hybrid promoter-PhaCAB-Bktb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 979
    Illegal BglII site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 1268
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 2199
    Illegal NgoMIV site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4065
    Illegal BsaI site found at 5108


Design Notes

This is a composite part, which developed based on the BBa_K2739008 (Btkb) and BBa_K1149051 (Hybrid-promoter-phaCAB).


Source

BBa_K2739008 (Btkb) used in the part was created through IDT. The original sequence would be accessed in NCBI: NC_015726.1.

Bktb sequence is codon optimised by IDT. BBa_K1149051 (Hybrid-promoter-phaCAB) is a available biobrick.

References

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