Tag

Part:BBa_K2719000

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 17:39, 16 October 2018 by AKARAM (Talk | contribs)


GST

Glutathione S-transferase (GST) is a peptide derived from Schistosoma japonicum, in which a target protein may be fused on the N-terminal. This fusion allows the purification of the target protein using glutathione affinity and maximize the presence of the protein in soluble state, rather than on inclusion bodies. In addition, this fusion protein gives stability to the domine of Tenascin V and for this is necessary to use a polyprolyne linker to maintain the original three dimensional structure of the protein.This tag may be later removed by adding a protease recognition site between GST and the target protein (Harper & Speicher, 2013).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None