Regulatory

Part:BBa_K2569006:Design

Designed by: wenxue Zhao   Group: iGEM18_SKLMT-China   (2018-09-27)
Revision as of 17:12, 16 October 2018 by DDY (Talk | contribs) (→‎Design Notes)


PfliA, constitutive promoter in Pseudomonas. fluorescence pf-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 62
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter PfliA is a strong constitutive promoter that can be used in regulating the gene expression in Pseudomonadaceae.

Source

Its sequence is taken from the upsteam of the gene fliA by artificially sythesis.

References