Device

Part:BBa_K2552007

Designed by: Zhixiang Liu   Group: iGEM18_SJTU-BioX-Shanghai   (2018-10-04)
Revision as of 16:12, 16 October 2018 by LZVX (Talk | contribs) (→‎Usage and Biology)


Pyear-azurin

This part is used to regulate azurin expression by Pyear promoter.


Usage and Biology

This part is used to regulate azurin(14kDa) expression by Pyear promoter. We add the His tag (5 Histidine amino acids) at the end of azurin protein in order to exam the expression of it. We insert the Pyear promoter and azurin protein into a regulative vector pCDFDuet1. After inducing with sedium nitrate, we extract all the protein in our strain and exam it by Western Blotting method. From the Fig1, we demonstrate that azurin protein successfully express with the His tag. 420px

https://parts.igem.org/File:T--SJTU-BioX-Shanghai--WesternBlotting.jpg<p/>

<img src="https://parts.igem.org/File:T--SJTU-BioX-Shanghai--WesternBlotting.jpg"/>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None