Coding

Part:BBa_K2665026

Designed by: Masaaki Shimazoe   Group: iGEM18_Kyoto   (2018-10-11)
Revision as of 15:58, 16 October 2018 by Sima914 (Talk | contribs)


SED1 anchoring domain

SED1 anchoring domain is domain derived from S.cerevisiae SED1 gene.
This domain is researched for cell-surface display of heterologous proteins. [1]
Using this part with SED1 secretion signal BBa_K2665025 , you can anchor objective protein on S.cerevisiae cell wall.
These are examples of design that include this part. BBa_K2665020 BBa_K2665021

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 325

Reference

[1]K. Inokuma, T.Bamba, J.Ishii et al. (2016) "Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide" Biotechnology and Bioengineering 2358-2366


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Parameters
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