Coding
Part:BBa_K2570019:Design
Designed by: Luying He Group: iGEM18_FJNU-China (2018-10-03)
tetR+GFP+mazF+PLtetO1+RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2590
Illegal AgeI site found at 2702 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have added an enzyme cutting site in the design to facilitate BIOBRICK.
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
Source
We ordered the part DNA from a synthesis company and had codon optimization of the sequence.