Composite

Part:BBa_K2680263:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)
Revision as of 15:00, 16 October 2018 by Ajoliver (Talk | contribs) (→‎Design Notes)


3G AraC-ssrA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 978


Design Notes

Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

To do

References